The "Legends of the Summer" tour will kick off at the Roger's Center in Toronto on July 17, and finish on August 16 at the Sun Life Stadium in Miami.
Venues in Boston, Detroit and Baltimore will also be included in the tour.
Earlier this week Live Nation said the duo, who together have won 23 Grammy awards and two Emmys and have sold 67 million albums, will also be performing together in London at the Wireless Festival on July 12-13.
Timberlake's new album, "The 20/20 Experience," which will be released next month, features "Suit & Tie," a collaboration with Jay-Z. The two performed a duet together at the Grammy Awards earlier this month.
(Reporting by Noreen O'Donnell; Editing by Patricia Reaney and Vicki Allen)